![](https://parts.igem.org/images/partbypart/icon_plasmid.png)
Plasmid
Part:BBa_K5000000:Design
Designed by: jian mo Group: iGEM23_Squirrel-Guangzhou (2023-10-11)
overexpression dxs, ispA, idi under the control of T7 promoter
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 3483
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 953
Illegal SpeI site found at 3483 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 3489
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 3483
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 3483
Illegal AgeI site found at 249 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1758
Design Notes
the three genes have independent RBS and the same T7 promoter
Source
all the coding sequence come from E.coli genomic sequence