Plasmid

Part:BBa_K5000000:Design

Designed by: jian mo   Group: iGEM23_Squirrel-Guangzhou   (2023-10-11)


overexpression dxs, ispA, idi under the control of T7 promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 3483
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 953
    Illegal SpeI site found at 3483
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 3489
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 3483
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 3483
    Illegal AgeI site found at 249
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1758


Design Notes

the three genes have independent RBS and the same T7 promoter


Source

all the coding sequence come from E.coli genomic sequence

References